Tài liệu Sinh học

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Evaluation of a multiplex PCR assay for rapid diagnosis of fowl typhoid

Despite Fowl typhoid (FT) eradication in commercial poultry of some parts of the world, outbreak threats are not eliminated in poultry of developing countries. In the current study, we reported molecular identification of Salmonella gallinarum in Indian isolates from backyard poultry by a multiplex PCR assay. Boiled culture lysates of Salmonella Gallinarum isolates (n=2) from an outbreak showed positive amplifications of glgC and speC genes in duplex PCR with 174 bp and 252 bp products, respectively. In multiplex PCR assay, primers for salmonella plasmid virulence gene (spvC) were also added in same reaction mixture. No amplifications were noticed in negative controls with other Salmonella serotypes and non –Salmonella organisms. This multiplex PCR assay was found to be rapid and specific in diagnosis of fowl typhoid.

3/29/2020 6:58:36 PM +00:00

Prevalence of pseudomonas aeruginosa in various clinical samples and its antibiotic susceptibility pattern in a Tertiary care hospital

Pseudomonas aeruginosa is one of the commonly isolated gram negative bacillus from various clinical samples. It is a commensal in healthy individuals but can be opportunistic pathogen especially in hospitalised patients. It is intrinsically resistant to many antibiotics. Hence the present study was undertaken to study the prevalence and antibiotic sensitivity pattern in our hospital. Various clinical samples like pus, sputum, urine, vaginal swab, pleural fluid, ear swabs and tracheal aspirate received in the microbiology laboratory were included in the study. Identification and antibiotic sensitivity testing was done by standard laboratory procedures. A total of 157 Pseudomonas aeruginosa were isolated from 858 clinical samples with a prevalence rate of 18.3%. Age and gender wise distribution shows higher prevalence rate in 40-60 years age group and in males. Maximum isolates were from pus, sputum and urine samples. Antibiotics tested were ceftazidime, ceftriaxone, piperacillin-tazobactum, aztreonam, amikacin, gentamycin, netilmicin, ciprofloxacin, ofloxacin, meropenem, cefixime. Antibiotic sensitivity pattern was found to show higher sensitivity to meropenem, piperacillin-tazobactum and amikacin. Resistance was more pronounced in cefixime, netilmicin, ceftriaxone and aztreonam. Spread of drug resistance in Pseudomonas aeruginosa is to be prevented by using antibiotics judiciously adhering to the antibiotic policy.

3/29/2020 6:58:00 PM +00:00